Abstract
Agar-diffusion assays with 10 different prokaryotic and eukaryotic strains were conducted to prove allelopathic activity of the cyanoprokaryote Anabaena torulosa. A. torulosa as well as five target organisms were isolated from lake Neusiedlersee (Austria). All indicator algae showed retardation when growing on plates inoculated with living material of A. torulosa. Filtrates of culture media as well as water extracts ofA. torulosa displayed no allelopathic effects indicating the absence of hydrophilic allelochemicals. Lipophilic methanol- and butylmethylether-extracts of A. torulosa showed bioactivity, the eukaryotic strain of Scenedesmus being more effected than the prokaryotic Microcystis. Semipreparative analysis by HPLC led to five fractions, three of them inhibiting growth of Scenedesmus.
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