Abstract
Calotropis procera (Ait.) Ait. is perennial medicinal obnoxious shrub growing in Pakistan up to 1500 m altitude. Hot and water aqueous extracts from leaves and young stems of C. procera were used against Pennisetum glaucum (Linn.) R. Br., Setaria italica (Linn.) P. Beauv., Brassica campestris Linn. and Lactuca sativa L. under laboratory condition. It was seen that germination, seedling growth, fresh and dry biomass reduced in concentration dependent manner. It was observed that the allelopathic effects depended upon the tested species, growth parameter measured, soaking duration and concentration of the donor plant material. The C. procera litter incorporated into the growth medium inhibited the test species used. The C. procera extracts from leaves were more inhibitory than stem extracts. The tendency of inhibition was radical growth > germination > plumule growth suggesting radicle growth to be a better measure of allelopathy. Leaf extracts significantly reduced division and size of cells. It is suggested that aqueous extract from C. procera can be further assessed against microbes and weed under laboratory and field condition.
Highlights
Calotropis procera (Ait.) Ait. (Family Asclepiadaceae) is an erect perennial obnoxious, non-palatable medicinal shrub found up to 1500 m altitude in drier parts of Pakistan
The original rain leachates were dilute; they were concentrated to 50% and 25% of original concentration in rotavapor. These three concentrations of rain leachates were tested against seeds and seedling growth of L. sativa and A. cepa in aqueous extract bioassay as above
Plant extract from leaves and stem-barks, prepared by soaking 5 g materials in 100 ml distilled water for 48 h, were tested as before against seeds of P. glaucum, S. italica, L. sativa and
Summary
Calotropis procera (Ait.) Ait. (Family Asclepiadaceae) is an erect perennial obnoxious, non-palatable medicinal shrub found up to 1500 m altitude in drier parts of Pakistan. Since some studies [1,2,3,4,5,6] on the allelopathy of Calotropis procera are available, the present study further envisaged its allelopathic stress against germination and seedling growth of some common crop species, and its effect on the division and development of cell as a possible allelopathic mechanism. This endeavor can be extended to identify its allelochemicals and phytochemicals responsible for the allelopathy
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