Allatostatin decreases stomatogastric neuromuscular transmission in the crab Cancer borealis.

Abstract

The effects of insect allatostatins (ASTs) 1-4 were studied on the stomach musculature of the crab Cancer borealis. Of these, Diploptera-allatostatin 3 (D-AST-3) was the most effective. D-AST-3 (10(-6 )mol l-1) reduced the amplitude of nerve-evoked contractions, excitatory junctional potentials and excitatory junctional currents at both cholinergic and glutamatergic neuromuscular junctions. Muscle fiber responses to ionophoretic applications of both acetylcholine and glutamate were reduced by the peptide, but D-AST-3 produced no apparent change in the input resistance of the muscle fiber. D-AST-3 reduced the amplitude of muscle contractures evoked by both acetylcholine and glutamate, but had no effect on contractures induced by a high [K+]. These data suggest that D-AST-3 decreases the postsynaptic actions of both neurally released acetylcholine and glutamate. Because an AST-like peptide is found in peripheral sensory neurons that innervate stomatogastric muscles and in the pericardial organs, we suggest that an AST-like peptide may play a role in controlling the gain of the excitatory neuromuscular junctions in the stomach.