Abstract
1 From the pH dependence of the rate of alkylation of pig heart malate dehydrogenase by iodoacetamide the pKa of the reactive histidine residue was determined as 7.1. 2 The enzyme is not inactivated by iodoacetic acid or 3-bromopropionic acid. 3 NADH protects the enzyme almost completely against alkylation by iodoacetamide whereas NAD, ADP-ribose and N-methylnicotinamide give only partial protection. 4 Oxaloacetate and malate do not protect against alkylation but mixtures of oxaloacetate and NAD or ADP-ribose protect completely, demonstrating formation of abortive ternary complexes.
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