Abstract
The levels and properties of liver alkaline phosphatase were compared in extracts from normal and Rich leukemia virus-infected mice. There was an increase in phosphatase activity in the livers from the virus-infected mice. This increase in enzyme activity was accompanied with alterations in the enzyme properties. The phosphatase activity in the extracts from infected mice was not as dependent on magnesium ions as was the enzyme from normal mice. Studies on acrylamide gel showed different electrophoretic mobilities for the alkaline phosphatase from the two sources. Heat stability studies showed that the Mg 2+-dependent species was more stable than the Mg 2+-independent species and that the extracts from infected mice contained a much lower concentration of this Mg 2+-dependent phosphatase than did the extract from normal mice. The apparent Michaelis constant, with respect to p-nitrophenyl phosphate, was larger (sevenfold) for the extract from the infected mice. These data show that leukemia virus infection of mice resulted not only in changes in the levels of activity, but also in changes in the properties of this enzyme.
Published Version
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