Alginate-based Microcapsules Facilitate Targeted Release of Lactobacillus plantarum LAB12 in vivo

Abstract

Event Abstract Back to Event Alginate-based microcapsules facilitate targeted release of Lactobacillus plantarum LAB12 in vivo Muhamad Fareez Ismail1, 2, 3, Siong Meng Lim1, 2 and Kalavathy Ramasamy1, 2* 1 Faculty of Pharmacy, Universiti Teknologi MARA (UiTM), Malaysia 2 Collaborative Drug Discovery Research (CDDR) Group, Universiti Teknologi MARA (UiTM), Pharmaceutical and Life Sciences Community of Research, Malaysia 3 Faculty of Dentistry, Mahsa University, Malaysia Background Microencapsulation can essentially provide probiotic lactic acid bacteria (LAB) a protective barrier against harsh gastrointestinal conditions. Although cell liberation in the intestines is deemed a mandatory prerequisite for effective application, in vivo validation of the delivery of LAB to the target site and subsequent digestibility of encapsulating matrix remains limited. Methods This study validated the release and fate of orally administered L. plantarum LAB12 encapsulated in a unique blend of alginate (Alg) and pea protein isolate (PPi) in Sprague Dawley rats (male; 4-6 weeks). Fate of the ingested microencapsulated LAB12 was examined at 1 and 4 hours post-ingestion in the stomach and the large intestines, respectively, by means of confocal imaging. The release of microencapsulated LAB12 were quantitatively assessed in terms of the proportion and relative abundance of theL. plantarum in various gut sections by qPCR. Results The microcapsules started to disintegrate in the large intestines 4 hours post-ingestion as evident by the presence of rod-shaped LAB12 and small pieces of polymeric matrix. qPCR confirmed that ingesta harvested from the stomach exhibited very minimal traces (<1 log CFU g-1) of L. plantarum which was unlikely associated with cell burst release from Alg-PPi microcapsules. Relative abundance of L. plantarum indicated an almost complete release of LAB12 from capsules after 16 hours post ingestion at target sites which included the ileum, caecum and colon. Conclusion Alg-PPi could serve as an ideal wall matrix that protects LAB12 from harsh gastric conditions and facilitate their release in the target site within the intestinal tract. Keywords: Lactobacillus plantarum LAB12, microencapsulation, Alginate, Pea protein isolate, Targeted release Conference: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019. Presentation Type: Poster Presentation Topic: Inflammatory diseases Citation: Ismail M, Lim S and Ramasamy K (2019). Alginate-based microcapsules facilitate targeted release of Lactobacillus plantarum LAB12 in vivo. Front. Pharmacol. Conference Abstract: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”. doi: 10.3389/conf.fphar.2019.63.00032 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 22 Oct 2018; Published Online: 17 Jan 2019. * Correspondence: Dr. Kalavathy Ramasamy, Faculty of Pharmacy, Universiti Teknologi MARA (UiTM), Bandar Puncak Alam, Malaysia, kalav922@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Muhamad Fareez Ismail Siong Meng Lim Kalavathy Ramasamy Google Muhamad Fareez Ismail Siong Meng Lim Kalavathy Ramasamy Google Scholar Muhamad Fareez Ismail Siong Meng Lim Kalavathy Ramasamy PubMed Muhamad Fareez Ismail Siong Meng Lim Kalavathy Ramasamy Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.