Abstract
A high level of enzymatic activity can be retained in tissues which have been fixed for very brief periods of time (5 to 10 minutes) by perfusion in aldehyde-DMSO solutions. Following the combination of short fixation time and the lead citrate method for alkaline phosphatase, membranes in the normal rat duodenum were studied.Tissues fixed in glutaraldehyde (4%), paraformaldehyde (4%), and a mixture of the two fixatives all containing 5% DMSO and 5% sucrose in a 0.1M cacodylate buffer were compared.
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