Abstract
Cell-free extract of a strain of Candida tropicalis cultivated on hydrocarbon catalyse in the presence of NAD + the oxidation of higher alcohols to the corresponding fatty acids; two enzymes, an alcohol dehydrogenase (alcohol:NAD + oxidoreductase, EC 1.1.1.1) and an aldehyde dehydrogenase (aldehyde:NAD + oxidoreductase, EC 1.2.1.3), have been studied. Assay of these enzymes (340-nm spectrometry) fails with substrates barely soluble in water. The assay is therefore carried out with emulsions prepared with Tween-80 or with deoxycholate. Alternatively the substrates were solubilized with dimethyl formamide. K m values obtained with 1-decanol using the modifications described were 31 mM, 7 mM and 0.7 mM, respectively. Kinetics properties and particularly the variation of the K m and ν max as a function of chain length of the substrates are reported. Alkane and the corresponding alcohol and aldehyde are inducers of these enzymes.
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