Aktywność olejku pichtowego (Oleum Pichtae) wobec bakterii beztlenowych

Abstract

Introduction. Abies whitebark (Abies sibirica L.) belonging to the family Pinaceae. The tree grown in Mongol, China and Siberian taiga. Produced the pichtae oil, which is obtained by hydrodistillation method. It contain: α-pinene, β-pinene, β-caryophyllene, bornyl acetate, camphene, mircene and cineole. The oil exhibiting expectorant, analgesic, anti-inflammatory, antialergic, liver restorative, adaptogenic and antioxidant properties. It has antimicrobial activity. Aim. The aim of the date was to determine the susceptibility of anaerobic bacteria isolated from patients to pichtae oil. Material and methods. The investigated 49 strains of bacteria isolated from patients from genus Bacteroides (7 strains), Parabacteroides (1), Prevotella (8), Porphyromonas (5), Tannerella (1), Fusobacterium (6), Finegoldia (4), Parvimonas (2), Peptostreptococcus (4), Actinomyces (4), Bifidobacterium (1), Propionibacterium (6), and 10 reference strains. The concentrations the oil were the following: 2.5, 5.0, 7.5, 10.0, 15.0 and 20.0 mg/ml. The pichtae oil was added to Brucella agar with 5% defibrynated sheep blood, menadione and hemin. Inoculum containing 106 CFU/ml was seeded with Steers replicator upon the agar with oil or without oil (strains growth control). The incubation was carried out in anaerobic jars containing 10% C02 , 10% H2 and 80% N2 , palladic catalyst and anaerobic indicator, at 37°C for 48 hrs. The MIC was defined as the lowest concentration of the pichtae oil that completely inhibited growth the anaerobic bacteria. Results. The results investigation indicated that from Gram-negative rods Tannerella forsythia (MIC = 5.0 mg/ml), Bacteroides fragilis and Bacteroides uniformis (MIC = 7.5 mg/ml) were the most susceptible to pichtae oil. The growth of Prevotella strains were inhibited by concentrations in ranges 5.0-15.0 mg/ml. The Prevotella bivia (MIC 10.0-15.0 mg/ml) and Prevotella buccalis (MIC = 15.0 mg/ml) were the most resistant. The tested oil was active on account genus of Fusobacterium strains in concentrations 5.0-10.0 mg/ml. The Gram-positive cocci were the more sensitive then rods. The growth was inhibited by concentrations in ranges ≤ 2.5-10.0 mg/ml. The oil was equally effective against Gram-positive rods (MIC ≤ 2.5-10.0 mg/ml). From this bacteria the more susceptible were the strains of Actinomyces (MIC ≤ 2.5-7.5 mg/ml) and the least a rods from genus of Bifidobacterium (MIC = 10.0 mg/ml). The date indicated, that the Gram-positive anaerobes were the more susceptible to pichtae oil than Gram-negative rods. Conclusions. From among the Gram-negative bacteria the more susceptible to pichtae oil were the rods from genus Tannerella forsythia, Bacteroides fragilis and Bacteroides uniformis. Gram-positive anaerobic cocci were the more susceptible then Gram-positive rods. The pichtae oil was the more active towards Gram-positive bacteria then Gram-negative anaerobic rods.