Aktivitas Ramuan Herbal sebagai Antagonis Reseptor H1, Relaksan Otot Polos dan Penghambat Degranulasi Sel Mast sebagai Antiasmatik

Abstract

Asthma is still become a major health problem worldwide, affected all ages from pediatrics to 
 adults. The severity of the disease vary from mild to severe and leads to the risk of death. The 
 uses of medicinal plants as antiasthmatic have often been conducted by the local community. 
 The present study was conducted in vitro and in vivo to determine the activities of herbs 
 containing of sembung leaves (Blumea balsamifera L.), kemukus seeds (Piper cubeba L.), teki 
 rhizome (Cyperus rotundus L.), and patikan kebo herbs (Euphorbia hirta L.) with certain ratio 
 as an antiasthmatic. The in vitro test was carried out on isolated smooth muscle cells of guinea 
 pig. Histamine was used to stimulate the contraction of smooth muscle cells of guinea pig 
 trachea. The in vivo test was carried out using guinea pigs which sensitized with ovalbumin 
 and administered with the herbs formula. The trachea of guinea pigs were prepared for 
 histopathological examination and stained on toluidine blue. The result revealed that the 
 antiasthmatic herbs of 1, 2, and 3 showed activities as H1 receptor antagonists. The 
 antiasthmatic herbs of 1, 2, and 3 were able to relax the smooth muscle of guinea pig’s trachea 
 that was experiencing contraction, but in lower level compared to theophylline. The
 antiasthmatic herbs formula in this study did not have the activity to inhibit mast cell 
 degranulation.