Abstract
Belimbing wuluh (Averrhoa bilimbi Linn.) is a tropical plant that has antibacterial properties. The purpose of this study was to test the antibacterial activity of bark Belimbing wuluh against Klebsiella pneumoniae and Staphylococcus epidermidis and their bioautography. Extraction methods used to research is method maceration with a solvent ethanol 96 %. Fractinations done by method partition liquid-liquid with a separating funnel. Test performed in this research covering identi� cation bacteria, the sensitivity bacteria, antibacterial activity, thin layer chromatography, bioautography. The result of antibacterial activity ethanol extract of disk diffusion method with concentrations 400 μg/disk, 800 μg/disk, 1600 μg/disk is 8±0,5; 10,34±0,58; 12,17±0,76 on Klebsiella pneumoniae, 10,17±0,29; 11±0; 11.5±0 on Staphylococcus epidermidis, n-hexane fraction with concentration 400 μg/disk, 800 μg/disk, 1600 μg/disk is 8,34±0,29; 9,34±0,29; 10,84±0,76 on Klebsialla pneumoniae, 8,5±0,5; 9,34±0,29; 10,67±0,29 on Staphylococcus epidermidis, ethyl acetate fraction with concentration 400 μg/disk, 800 μg/disk, 1600 μg/disk is 9,17±0,29; 10,34±0,29; 11,17±0,29 on Klebsiella pneumoniae and 9,5±0,5; 10,67±0,29; 12,67±1,26 on Staphylococcus epidermidis, ethanol-water fractions with concentration 400 μg/disk, 800 μg/ disk, 1600 μg/disk is 8,17±0,29; 9,17±0,29; 10±0 on Klebsiella pneumoniae, 9±0; 9,67±0,29; 10,34±0,29 on Staphylococcus epidermidis. The TLC show chemical compounds contained in the ethanol extract, n-heksan fraction, ethyl acetate fraction, and ethanol-water fraction is a compound of the saponins, alkaloids, � avonoids and phenolic. Bioautography showed that ethanol extracts, n-heksan faction, ethyl acetate fraction, and etanol-airfaction Belimbing wuluh (Averrhoa bilimbi Linn.) bark have not antibacterial activity because there is no clear area around on plate TLC.Keywords: Belimbing wuluh (Averrhoa bilimbi Linn.), ethanol extract, fractination, antibacterial, bioautogra� .
Highlights
PENDAHULUAN Infeksi merupakan penyakit yang sering di jumpai masyarakat di seluruh dunia
The purpose of this study was to test the antibacterial activity of bark Belimbing wuluh against Klebsiella pneumoniae
Fractinations done by method partition
Summary
Ekstraksi dan fraksinasi Ekstraksi dilakukan untuk mendapatkan zat aktif berkhasiat. Maserasi tergolong metode yang mudah dilakukan dan peralatan yang digunakan sederhana. Pelarut yang digunakan untuk fraksinasi adalah n-heksan, elil asetat, dan etanol-air. Metode yang digunakan pada penelitian ini adalah partisi cair-cair dengan menggunakan corong pisah. Pemilihan fraksinasi dengan corong pisah dilakukan karena peralatannya sederhana dan tidak membutuhkan waktu yang lama. Hasil yang diperoleh fraksi n-heksan 3,24 g dengan rendemen 16,2%, etil asetat 2,91 g dengan rendemen 14,55%, dan etanol-air 12,25 g dengan rendemen 16,25%. Aktivitas Antibakteri dengan Metode Difusi Pengujian dilakukan untuk mengetahui khasiat antibakteri dari kulit batang belimbing wuluh (Averrhoa bilimbi L.) terhadap bakteri Klebsiella pneumoniae dan Staphylococcus epidermidis. Metode untuk uji aktivitas antibakteri adalah cara Kirby Bauer (Metode disc diffusion) dengan media Mueller Hinton (MH). Pemilihan metode Kirby Bauer pada uji aktivitas antibakteri karena pengerjaannya sederhana. Hasil uji aktivitas antibakteri ekstrak, fraksi n-heksan, fraksi etil asetat, dan etanol-air terhadap bakteri Klebsiella pneumoniae dan Staphylococcus epidermidis
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