Aislamiento y caracterización de nuevos marcadores microsatélites para la almeja generosa (Panopea generosa ) por medio de secuenciación de nueva generación

Abstract

Panopea generosa is a large and long-lived infaunal clam with a considerable commercial value in Canada, United States and Mexico. Here, we tested 30 microsatellite loci generated using next-generation genome sequencing (Illumina Hi-Seq 2500) and identified eight as suitable polymorphic genetic markers. The number of alleles per locus ranged from 5 to 22 and heterozygosity from 0.429 to 0.818 (observed) and from 0.548 to 0.962 (expected). These markers are highly informative and useful for population genetic studies aimed at informing management and conservation measures of this valuable resource.