Air pollution by SO2 amplifies the effects of water stress on enzymes and total soluble proteins of spruce needles

Abstract

Measurements of soluble protein levels and catalytic capacity (maximum extractable activity) of isocitrate, glucose‐6‐phosphate and glutamate dehydrogenases were performed in needles of Picea abies L. Karst. under phytotron‐controlled conditions in filtered or SO2 polluted (0.08 ppm, 3.1 μmol m−3) air. In watered plants, pollution had no significant effects, although sulphur accumulated in the needles. Water deprivation (1 or 2 weeks depending on the experiments) of non‐polluted plants decreased protein concentration and modified enzyme capacity, particularly for isocitrate and glucose‐6‐phosphate dehydrogenases. These effects were amplified in the polluted plants. Visible damage occurred only in plants subjected to both pollution and water stress. The results indicate that in spruce needles vulnerability of cell metabolism to the effects of a drought period is increased when water deprivation occurs under SO2 pollution.