Abstract
Stevia rebaudiana accumulates steviol glycosides (SGs) that are used as noncaloric, natural sweeteners in food industry. The most important SGs are rebaudioside A (Reb A) and stevioside (St), and they are markers of the quality of S. rebaudiana lines. In this work, the production and physiology of SGs in Agrobacterium rhizogenes-transformed plantlets were analyzed. By means of HPLC–DAD, the production of St and Reb A was quantified, resulting in a 1.4- and 1.5-fold production increase of St and Reb A, respectively, in transformed compared to wild-type plantlets. Superior phenotype of transformed plantlets was observed in comparison with wild type, represented as an increase of 25% in biomass of aerial parts, 43% in biomass of roots, 20–30% of leaf area and 24% of chlorophylls content. Moreover, the SG production profiles evaluated in a 20-d period showed higher yield in the transformed line. The results of this work demonstrated the usefulness of S. rebaudiana transformation via A. rhizogenes as a strategy to explore new approaches for the improvement of SGs production.
Published Version
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