Abstract
Abstract Stem explants of in vitro-propagated peach (Prunus persica L. Batsch.) cultivars Compact Redhaven, Jerseyqueen, Rio Oso Gem, and Suncrest and peach rootstock Nemaguard were infected with several strains of Agrobacterium tumefaciens. Tumor tissue was produced on all cultivars infected with strain tms328::Tn5, which carries a mutated octopine Ti plasmid pTiA6. The tumor tissues were cytokinin-independent and all tissues, except those on ‘Rio Oso Gem’, produced octopine. A DNA probe specific for the T-DNA region of the Ti plasmid hybridized to the DNA extracted from these tumors. No tumor tissue was produced in response to inoculation of the stem explants with the Ti-plasmidless strain A136 or with uninoculated controls. These results demonstrate the transformation of peach cells derived from mature plants and the potential for using A. tumefaciens to transfer economically important genes to peach.
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