Agro-industrial byproducts as modification enhancers of the bacterial cellulose biofilm surface properties: An inverse chromatography approach

Abstract

Bacterial cellulose (BC) has remarkable and excellent properties. However, the high-cost production and the use of chemicals for BC modification make its application unattractive. A simple approach to modify the properties of BC during biosynthesis, by using alternative low-cost carbon sources from agro-industrial byproducts, was studied. The carbon source (date syrup, mannitol, sucrose, food-grade sucrose, and glucose) significantly changed the BC network morphology and structure. Date syrup source induces the highest modifications in the surface properties of BC: smaller area (SBET = 4.04 m2/g), higher hydrophobic (γsd = 45.79 mJ/ m2) and basic character (Kb/Ka = 1.10), at 25 ºC. Food-grade sucrose source resulted in the lowest yield of BC production (37% less), however caused an increase in the BC network reticulation and a high crystalline structure (IC = 82.3%). This sustainable and simple methodology presents a low-cost and efficient approach allowing the modulation of the surface properties of BC.