Aging of mesenchymal stem cell in vitro

Mandana Mohyeddin Bonab,Behrouz Nikbin,Syed Hamid Ghaffari,Kamran Alimoghaddam,Ardeshir Ghavamzadeh,Fatemeh Talebian

doi:10.1186/1471-2121-7-14

Mandana Mohyeddin Bonab, Behrouz Nikbin + Show 4 more

Open Access

https://doi.org/10.1186/1471-2121-7-14

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Abstract

BackgroundA hot new topic in medical treatment is the use of mesenchymal stem cells (MSC) in therapy. The low frequency of this subpopulation of stem cells in bone marrow (BM) necessitates their in vitro expansion prior to clinical use. We evaluated the effect of long term culture on the senescence of these cells.ResultsThe mean long term culture was 118 days and the mean passage number was 9. The average number of PD decreased from 7.7 to 1.2 in the 10th passage. The mean telomere length decreased from 9.19 Kbp to 8.7 kbp in the 9th passage. Differentiation potential dropped from the 6th passage on. The culture's morphological abnormalities were typical of the Hayflick model of cellular aging.ConclusionWe believe that MSC enter senescence almost undetectably from the moment of in vitro culturing. Simultaneously these cells are losing their stem cell characteristics. Therefore, it is much better to consider them for cell and gene therapy early on.

Highlights

A hot new topic in medical treatment is the use of mesenchymal stem cells (MSC) in therapy
The fibroblast like cells (MSC) increased and the heterogeneous cells decreased in the culture
Since MSC can differentiate into multiple tissues in vitro and in vivo, they attract a lot of attention in cell and gene therapy

Summary

Introduction

A hot new topic in medical treatment is the use of mesenchymal stem cells (MSC) in therapy. Mesenchymal stem cells (MSC) are of great therapeutic potential because of their ability to self-renew and differentiate into multiple tissues[1]. They enhance engraftment of donor hematopoietic cells after cotransplantation in animal models[2]. We estimated that about 1 in 3.1 × 104 bone marrow mononuclear cells are MSC (unpublished data). Their level is even lower in cord blood and peripheral blood. In vitro culture has proven that environmental conditions and differing treatment significantly increase or decrease culture life span It seems as though depending on conditions cells (page number not for citation purposes)

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