Aging-dependent brain electrophysiological effects in rats after distinct lactation conditions, and treadmill exercise: A spreading depression analysis

Abstract

Aging-related neurophysiological alterations are a matter of growing concern in gerontology. Physical exercise has been therapeutically employed to ameliorate aging-associated deleterious neurological changes. The aging process, as well as the effects of treadmill exercise on brain excitability, can be influenced by nutritional demands during lactation. In this study we investigated whether physical exercise, lactation conditions, and aging interact and modulate brain electrophysiology as indexed by the excitability-related phenomenon known as cortical spreading depression (CSD). Wistar male rats were suckled in litters of 12 or 6 pups (constituting two groups named L12 and L6), with different lactation conditions. Each group was subdivided into exercised (treadmill) and sedentary. CSD was recorded immediately after the exercise period for young, adult, and aged groups (respectively 45–60, 120–130, and 600–700days old). In L6 groups, the mean CSD velocity (in mm/min) ranged from 2.57±0.24 in aged rats to 3.67±0.13 in young rats, indicating an aging-related CSD deceleration. The L12 condition accelerated CSD (velocities ranging from 3.11±0.21 to 4.35±0.16 in aged and young rats, respectively) while treadmill exercise decelerated it in both L6 groups (range: 3.02±0.19 to 2.57±0.24) and L12 groups (3.32±0.16 to 3.11±0.21), with an observed interaction between factors in the aged group. Furthermore, aging led to a significant failure of CSD propagation. These results contribute to the understanding of underlying mechanisms by which exercise and aging influence brain electrophysiological functioning, previously associated with distinct lactation conditions during the period of brain development.