AGE-RELATED CHANGES IN CELL SURFACE MARKERS IN BALB/C MICE: A FLOW CYTOMETRIC ANALYSIS

Abstract

Event Abstract Back to Event AGE-RELATED CHANGES IN CELL SURFACE MARKERS IN BALB/C MICE: A FLOW CYTOMETRIC ANALYSIS EMINE YAVUZ1, SUHEYLA HASGUR1 and EMIN U. BAGRIACIK1* 1 GAZI UNIVERSITY SCHOOL OF MEDICINE, IMMUNOLOGY, Turkey Introduction: Age-related phenotypic alterations have been indentified in lymphocytes. However, the effect of aging on lymphocyte surface markers in BALB/c mice was not studied inclusively and systematically in a single study. The aim of this study is to analyze and identify the cell surface markers of lymphocyte populations from various lymphoid organs of young and aged mice. Material and Methods: Spleen, lymph nodes, mesenteric lymph nodes, thymus, and intraepithelial T lymphocytes were isolated from BALB/c mice. Flow cytometric analysis of lymphocytes was performed by using cell surface receptor spesific labelled antibodies. Results: The CD4:CD8 ratio has declined with age in thymus. In spleen, while the number of CD4+CD45R+, CD4+CD28+, CD4+CTLA4+, and CD8+CD28+, NKpan+ cell populations have increased with age, TCRαβ+ and CD62L+ cell counts have decreased. In lymph nodes, CD4+CTLA4+, CD8+CD45RB+, CD8+CD45R+, CD11b+ , and CD11c+ cell numbers have increased with age but the number of CD8+CD28+, CD4+CD45RB+ cells have declined. Partially different results was seen in mesenteric lymph nodes: CD4+CD8-, CD4+CD25+, CD8+CD25+, CD8+CD28+, CD62L+, and CD11b+ cell counts were less in older mice. In intestinal intraepithelial lymphocytes, the number of TCRγδ+ cells has decreased with increasing age while CD4+CD8α+ cell count has increased. Discussion: The changes in cell surface markers of T cells of lymphoid organs and mucosal lymphoid tissues in BALB/c mice are crucial during aging. The number of innate immune system cells also differs between young and old BALB/c mice. These results might be important to understand the physiological process of immune senescence and immune regulatory mechanisms of aging immune system. Keywords: Immune senescence, Balbc mice, Flow Cytometry, Lymphocytes, IEL Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune receptors and signaling Citation: YAVUZ E, HASGUR S and BAGRIACIK EU (2013). AGE-RELATED CHANGES IN CELL SURFACE MARKERS IN BALB/C MICE: A FLOW CYTOMETRIC ANALYSIS. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00199 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 10 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Prof. EMIN U BAGRIACIK, GAZI UNIVERSITY SCHOOL OF MEDICINE, IMMUNOLOGY, ANKARA, Turkey, eubagriacik@yahoo.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers EMINE YAVUZ SUHEYLA HASGUR EMIN U BAGRIACIK Google EMINE YAVUZ SUHEYLA HASGUR EMIN U BAGRIACIK Google Scholar EMINE YAVUZ SUHEYLA HASGUR EMIN U BAGRIACIK PubMed EMINE YAVUZ SUHEYLA HASGUR EMIN U BAGRIACIK Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.