Abstract
Estimation of age dependent changes of the lipid peroxidation (LPO) intensity, content of stable metabolites of nitrogen oxides (NO) and antioxidant enzyme activities in rat blood serum in conditions of experimental postinfarction cardiosclerosis (PICS) is carried out. Initiation of the postinfarction remodeling of animals has been carried out with coronary occlusion, definition of LPO and NO metabolites indices has been performed after 45 days. Investigations have been carried out on 40 four and twelve-month-old male Wistar rats with mass 200-250 gand 400 -450 g, accordingly. Statistical analysis of the results was performed using the Mann-Whitney-Wilcoxone criterion. It has been found that already intact animals have age specificity of indices under consideration. The expressed activity of LPO processes on the background of reduction of endogenous fermentative antioxidant (SOD and catalase) activity as well as nitrite concentration in blood serum is characteristic for PICS of 4-month-old animals. PICS of 12 month-old rats is accompanied with suppression of the LPO processes on the back- ground of reduction of the antioxidant enzyme intensity and increase of NO metabolites production. The following conclusions have been drawn. Process of ontogenesis is characterized by imbalance between pro-and antioxidant processes in rat blood. Increase in catalase activity and concentration of the TBC-active products at simultaneous decrease of SOD activity and content of diene conjugates has been noted. The organism of young animals responds with persistent increase of LPO processes and decrease of SOD and catalase activity on formation of postinfartion cardioslerosis. The less expressed increase of lipid peroxidation activation and decrease of catalase activity has been noted in the organism of old animals within 45 day after PICS formation.
Highlights
Free radical reaction has a definite place in the processes that occur in the alive organisms
It has been found that statistically significant increase of TBA-active products (TBAAP) on 25% and decrease of DC content on 20% is characteristic for the control twelve-month-old animals in comparison with the young animal group (Table 1)
The revealed age dependent differences in TBAAP and DC concentration and Superoxide dismutase (SOD) and catalase activity can be reflected on change of these indices at development of DC, ΔЕ232/ml one of the aging mechanism of organism [9,10]
Summary
Free radical reaction has a definite place in the processes that occur in the alive organisms. The main source of radicals in organism is oxygen. Change of lipid peroxidation intensity in aging process of organism has been found [5,9,10]. The free radicals are very aggressive molecules which enter in interaction with the main cellular components causing oxidative modification of lipids, thiol groups of proteins and nucleic acids [6]. Oxidative modification of molecules leading to structural disturbance in the membrane bilayer and decrease of functional activity of integral membrane proteins is the pathogenetic link of the many pathological processes [6,11]. At the same time the clear idea about intensity of processes of lipid peroxidation (LPO) and their pathogenetic role
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
