Abstract
To investigate and correlate total AgNOR area/Total nucleus area (TAA/TNA) values in buccal epithelial cells of healthy individuals in different age groups. In present study 50 healthy individuals are included with age ranging 10 to 60 years. These are divided into 5 groups. Group 1-10-20 year, Group2-20- 30year, Group3-30- 40year, Group 4-40-50-year, Group 5-50-60 year. Oral epithelial cells collected with the help of cyto brush from buccal mucosa. Smears were prepared on clean glass slides and fixed with 95% alcohol. Fixed slides Smears were stained with AgNOR stain. Statistically significant correlations were found between mean TAA/TNA values and age p < 0.001 for linear and p < 0.0001 for polynominal regression, and between AgNOR number and age, p < 0.001 for linear. There is a significant correlation between age and AgNOR amount (ribosome biosynthesis rate) in buccal epithelial cells of healthy individuals. AgNORs in buccal epithelial cells may be used for detection of age
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