Abstract
An agarose electrophoresis system in veronal buffer at pH 6.5 was developed to permit fast isozyme separations on subtilopeptidase A (Carlsberg subtilisin [EC 3.4.4.16]). This system was designed for application in crossed immunoelectrophoresis, according to Laurell and Freeman. The anode vessel contains phosphate buffer. At the cathode a vessel with running buffer is connected via a liquid bridge to a heated saturated solution of veronal acid. The improvement in speed over conventional buffers is shown. The value is demonstrated in the possibility to reveal batch to batch differences. The effect of DFP inhibition on the electrophoresis pattern is discussed.
Published Version
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