AFLP and SRAP markers linked to the mj gene for root-knot nematode resistance in cucumber

Zübeyir Devran,Ibrahim Halil Elekçioğlu,Ahmet Fikret Firat,Nedim Mutlu,Mahmut Tör

doi:10.1590/s0103-90162011000100017

Zübeyir Devran, Ibrahim Halil Elekçioğlu + Show 3 more

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https://doi.org/10.1590/s0103-90162011000100017

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Journal: Scientia Agricola	Publication Date: Feb 1, 2011
Citations: 27	License type: cc-by

Affiliation: Akdeniz University, University of Warwick

Abstract

Root-knot nematodes (Meloidogyne spp.) are an important worldwide pest of cucumber (Cucumis sativus L.). Molecular markers linked to the Javanese root-knot nematode (M. javanica) resistance gene mj in cucumber may aid marker assisted selection. One-hundred AFLP (EcoRI-MseI) and 112 SRAP were used to screen resistant and susceptible parents for polymorphisms to develop molecular markers linked to the mj gene. Of the 100 AFLP primers, 92 produced bands and two yielded candidate markers (E-ATT/M-CAA and E-AAC/M-CTG). These two bands were cut off from polyacrylamide gel, cloned and sequenced. Primers designed from the sequences did not yield polymorphic bands between the parents. In addition, the sequences did not contain any restriction site or indel to be used to convert them to CAPS or SCAR markers. The two sequences obtained from polymorphic AFLP markers were used primarily to design D1F, D1R, D17F and D17R primers. SRAP forward and reverse primers were used in combination with these four specific primers to search for polymorphisms between parents. Of the 112 primer combinations 11 yielded polymorphisms between parents. MapMaker Exp 3.0 software was used to analyze the 11 markers. Two markers were identified that flanked the mj gene at distance of 16.3 and 19.3 cM. The results indicated that these markers should be useful to develop molecular markers flanking the mj gene.

Highlights

Cucumber (Cucumis sativus L.) is highly susceptible to root-knot nematodes (Meloidogyne spp.) and often reduces the productivity of this crop (Fassuliotis, 1979; Netscher and Sikora, 1990; Wehner et al, 1991). Fassuliotis (1982) reported that four economically important root-knot nematode species
Many cultivars and wild cucumber accessions have been evaluated for resistance to root knot nematodes (Fassuliotis and Rau, 1963; Fassuliotis, 1967; Norton and Granberry, 1980; Winstead and Sasser, 1956; Walters et al, 1990; Wehner et al, 1991; Walters et al, 1992; Walters et al, 1993; Walters et al, 1996; Walters et al, 1999)
Resistance to root knot nematodes has been identified in Cucumis metuliferus (Norton and Granberry, 1980; Wehner et al, 1991)

Summary

Introduction

Cucumber (Cucumis sativus L.) is highly susceptible to root-knot nematodes (Meloidogyne spp.) and often reduces the productivity of this crop (Fassuliotis, 1979; Netscher and Sikora, 1990; Wehner et al, 1991). Fassuliotis (1982) reported that four economically important root-knot nematode species Many cultivars and wild cucumber accessions have been evaluated for resistance to root knot nematodes (Fassuliotis and Rau, 1963; Fassuliotis, 1967; Norton and Granberry, 1980; Winstead and Sasser, 1956; Walters et al, 1990; Wehner et al, 1991; Walters et al, 1992; Walters et al, 1993; Walters et al, 1996; Walters et al, 1999). Walters et al (1997) reported the gene conferring resistance to M. javanica as a single recessive gene, mj This gene was introgressed from C. sativus var. Hardwicki line LJ90430 into cultivated cucumber and inbred cultivars (Monteo, Shelby, and Lucia) were developed that are resistant to M. arenaria races 1 and 2, and M. javanica (Walters and Wehner, 1997) This gene was introgressed from C. sativus var. hardwicki line LJ90430 into cultivated cucumber and inbred cultivars (Monteo, Shelby, and Lucia) were developed that are resistant to M. arenaria races 1 and 2, and M. javanica (Walters and Wehner, 1997)

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