Abstract

A total of 178 milk samples (94 of buffalo and 84 of cow) were randomly taken from Punjab and the North West Frontier Province (NWFP) of Pakistan (n = 89 in each province) and analysed for the presence of aflatoxin M1 (AFM1) by HPLC-FLD. From Punjab about 46% of buffalo's and 49% of cow's milks were contaminated with AFM1 as compared with 52% and 51% for milk samples from NWFP, respectively. Overall, the mean AFM1 concentration was 0.046 µg kg−1 with a maximum of 0.350 µg kg−1. All samples complied with the Codex Alimentarius limit of 0.50 µg kg−1 for AFM1 in milk, but 16.3% of samples exceeded the European Union maximum level of 0.05 µg kg−1. Another set of 415 buffalo's and cow's milk samples (213 morning milks and 202 evening milks) were analysed. Statistical analysis revealed significant differences (p < 0.05) between mean AFM1 concentrations in milk during the morning (0.043 µg kg−1) and the evening (0.028 µg kg−1) lactation times.

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