Abstract
Affinity partition of yeast and horse liver alcohol dehydrogenases (ADH) in two-phase systems containing polyethylene glycol (PEG)-dyes is most effective using Cu(II) complexes of Light resistant yellow 2KT and Red-violet 2KT. The effects of NaCl, NAD and chelating agents (imidazole and adenine) on the partitioning of ADH's were studied. It was shown that two-phase systems containing dyes in the top and bottom phases with different affinity to yeast ADH are promising for the extraction of enzyme from crude extract.
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