Aerobic Training Activates Nrf 2 -mediated Anti-oxidative Effect Of Ampk On Mouse Skeletal Muscle

Abstract

PURPOSE: To study the antioxidative effect of AMPK activated by aerobic training on Nrf2 mediated skeletal muscle in mice. METHODS: 8-week-old AMPK α 2 transgenic mice (TG mice), AMPK α 2 knockout mice (KO mice) and wild-type mice (WT mice) were randomly divided into two groups: the quiet group and the training group, with 10 mice in each group. In the training group, 6 days a week, aerobic training was conducted on the running platform with a gradient of 0 and a speed of 12 m / min for 1 hour every day for 4 weeks. The level of ROS in skeletal muscle was detected by fluorescence colorimetry. Rc-pcr was used to detect the level of antioxidant enzyme mRNA in skeletal muscle of mice in each group. Western blot method was used to detect the expression of AMPK α 2, AMPK α, Nrf2, pnrf2 and antioxidant enzyme protein. RESULTS: after 4 weeks of aerobic training (3) the expression of pnrf2 protein in skeletal muscle of wild mice and AMPK α 2 transgenic mice increased significantly. (4) compared with the wild mice, the expression of AMPK α 2 gene knockout mice skeletal muscle catmrna, nqo-1mrna, ho-1mrna, ho-1mrna, gpx-1mrna and sod1mrna decreased significantly. (5) the expression of cat, GSR, HO-1 and SOD1 protein in the skeletal muscle of wild mice increased significantly. The expression of AMPK α 2 in transgenic mice was high, and the expression of protein (cat, GPx-1, nqo-1) and protein (GCLC, GSR, HO-1, SOD1, SOD2) in the skeletal muscle of wild mice increased significantly. Compared with the wild mice in the training group, the expression of AMPK α 2 in transgenic mice was high (GPx-1, GSR, HO-1, nqo-1, SOD1) , SOD2) and GCLC protein expression increased significantly. Compared with the wild mice in the training group, AMPK α 2 knockout mice showed a significant decrease in the expression of protein (HO-1 and SOD2) in skeletal muscle tissue. (6) there was no significant difference in ROS level in skeletal muscle of wild mice, AMPK α 2 transgenic mice and AMPK α 2 knockout mice before and after training. CONCLUSIONS: the activation of AMPK by aerobic training can promote the expression of pnrf2 in skeletal muscle, then increase the expression of antioxidant enzymes and improve the antioxidant capacity of the body.