AEG-1 expression correlates with CD133 and PPP6c levels in human glioma tissues.

Guo Jia,Zhang Xuanwei,Chen Xin,Xi Ruxing,Chang Yuwei,Zhang Xiaozhi

doi:10.7555/jbr.28.20140015

Abstract

Astrocyte elevated gene-1 (AEG-1) is associated with tumor genesis and progression in a variety of human cancers. This study aimed to explore the significance of AEG-1 in glioma and investigate whether it correlated with radioresistance of glioma cells. Immunohistochemical staining showed that the intensity of AEG-1, CD133 and PPP6c protein expression in glioma tissues increased significantly, mainly in the cytoplasm. The expression rate of AEG-1, CD133 and PPP6c were 85.9% (67/78), 60.3% (47/78) and 65.8% (51/78), respectively. AEG-1 expression was correlated with age (r = 0.227, P = 0.045), clinical stage (r = 0.491, P<0.001) and clinical grade (r = 0.450, P<0.001). No correlation was found between AEG-1 expression and other clinicopathologic parameters (P>0.05). The expression of AEG-1 was positively correlated with the expression of CD133 (r = 0.240, P = 0.035) and PPP6c (r = 0.250, P = 0.027). In addition, retrieved data on TCGA implied co-occurrence of genomic alterations of AEG-1 and PPP6c in glioblastoma. Our findings indicate that AEG-1 is positively correlated with CD133 and AEG-1 expression. It may play an important role in the progression of glioma and may serve as potential novel marker of chemoresistance and radioresistance.

Highlights

Malignant glioma is the most common primary brain tumor in adults and one of the most deadly and least successfully treated solid tumors
To determine whether Astrocyte elevated gene-1 (AEG-1) is related to cancer stem cell or cell radioresistance in glioma, we examined the correlation of AEG-1 and a glioma stem cell (GSC) marker CD133 and glioma cell radioresistance maker PPP6c (Fig. 1)
Our study demonstrated that AEG-1 was widely expressed in many histological types of glioma, and correlated with clinical course and the histological grade, as previously reported[28,29]

Summary

Introduction

Malignant glioma is the most common primary brain tumor in adults and one of the most deadly and least successfully treated solid tumors. Despite rapid advancement of multimodal treatments for patients afflicted with glioma, the prognosis has not been significantly improved, and the median survival for patients with malignant glioma is less than 1.5 years[1]. The modest increase in survival after radiotherapy has been ascribed to the high intrinsic resistance of malignant glioma to ionizing radiation (IR). Previous studies have shown that many biological characteristics of tumor cells were associated with AEG-1 expression[2]. Lee et al reported[3] that AEG-1 could increase Akt phosphorylation, promote FOXO3a translocation out of the nucleus and inhibit apoptosis. AEG-1 is closely associated with tumor metastasis.

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