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PDF Availablehttps://doi.org/10.1016/j.fsigss.2015.09.205
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Cite Publication Date: Oct 2, 2015 |
Affiliation: Netherlands Forensic Institute
We noticed variation in the level of background noise when using custom-made fluorescently labelled primer mixes during PCR amplification. Various aspects that may affect the level of noise generated during PCR set-up were assessed. Best results are obtained when the multiplex buffer is added as a last component.
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