Advancements of fermentable sugar yield by pretreatment and steam explosion during enzymatic saccharification of Amorphophallus sp. starchy tuber for bioethanol production

Abstract

The present investigation focuses on creating substantial fermentable sugars from tubers obtained from the northern region of Thailand to advance bioethanol production. The generation of bioethanol from tubers was assessed to chemical and biological pretreatment advancements. The enzymatic pretreatment can significantly improve biomass scarification to boost biogenic ethanol production. The result reflected that after the enzymatic hydrolysate the maximum fermentable sugars of Amorphophallus spp. tuber via ethanolic fermentation can be achieved up to 19.01 g/L, where the S. cerevisiae is subjected as inoculum. In addition, the total sugar concentration was 33.22 g/L under optimum conditions. The maximum ethanol level, 8.68 ± 0.91 g/L, was produced after 48 h of alcoholic incubation employing S. cerevisiae. The ethanol heat power obtained from the Amorphophallus spp. was estimated at 24.65 ± 0.159 MJ/kg, above the average of 16.6 to 21.2 MJ/kg. Conclusively, the enzymatic saccharification help to boost the hydrolysis activity by changing the composition characteristics of biomass and improves the availability of reducing sugar which finally increases bioethanol production.