Abstract
The electrochemical response of vitamin B1 at different types of carbon electrodes viz: glassy carbon electrode (GCE), carbon paste electrode (CPE), single walled carbon nanotube paste electrode (SWCNTPE) and multi walled carbon nanotube paste electrode (MWCNTPE) has been investigated using cyclic voltammetric (CV) and differential pulse adsorptive stripping voltammetric (DPAdSV) methods. A simple and selective differential pulse adsorptive stripping voltammetric method has been developed for the determination of vitamin B1 in pure form and in pharmaceutical preparations. Various parameters that can influence the peak signal (effect of buffer, accumulation time, pH, scan rate, accumulation potential, and pulse amplitude) have been scrutinized. The best results were obtained in acetate buffer (pH 6.3) using multi-walled carbon nanotube paste electrode at a deposition time of 80s, deposition potential of 0.0V, scan rate of 50mVs−1 and pulse amplitude of 50mV.The reduction peak current varies linearly with the concentration of vitamin B1 over the range of 1.0×10−7–1.0×10−6M. The limits of detection and quantification of the pure drug are 1.1×10−10M and 2.0×10−9M, with the correlation coefficient, r=0.997 and the relative standard deviation, RSD=1.2% (n=5). Experimental results reveal that the MWCNTPE has advantages of small background current, high signal to background current ratio and good reproducibility.
Published Version
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