Abstract
The immobilization of paramecia when mixed with appropriate antisera has been utilized as a specific and semi� quantitative assay for the serotypic antigens (adsorption titration). While serotypic antigen in both soluble and particle�bound forms is detected by adsorption titration, only soluble antigen is detected in gel.diffusion analysis. 500 g, 3000 g, 12,000 g, and 105,000 g particulate fractions as well as 105,000 g and 150,000 g cell supernatant fractions were prepared by differential centrifugation. Analyses of these fractions indicate:
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