Abstract

Adsorption of lysozyme on the mesoporous carbons with various pore diameters from buffered solution at various pHs has been studied. The amount of adsorbed lysozyme depends on the solution pH as well as on the specific pore volume and pore diameter of the adsorbents. The maximum adsorption was observed near the isoelectric point of the lysozyme (pI≈11), suggesting that suppression of electric repulsion between the enzymes plays an important factor in the adsorption process. The unaltered structural order of the mesoporous adsorbents after the adssorption at different pH has been confirmed by the physico-chemical characterization techniques such as XRD and N2 adsorption. In addition, FT-IR spectroscopic studies confirmed that the lysozyme used in this study is stable even after the adsorption on the mesoporous carbon. These results indicate that mesoporous carbon adsorbent can be one the best media for the biomaterials adsorption.

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