Abstract
This study deals with developing bentonite–tryptophan (Bent–Trp) microcomposite affinity sorbent (38–105μm) for lysozyme adsorption from aqueous solutions. The structural analysis results of Bent–Trp microcomposites obtained from X-ray diffraction (XRD), Fourier Transform Infrared (FTIR), thermal (TG-DTG/DTA) and elemental analysis techniques clearly pointed out that the pseudo-biospecific affinity ligand L-Tryptophan was penetrated into interlayer space of the bentonite by diplacing water molecules and an elemental analysis of immobilized L-Tryptophan for nitrogen was 541.3μmolg−1 bentonite. The effects of initial concentration, pH, and temperature on the adsorption efficiency of this sorbent were also studied in a batch system. The maximum amount of lysozyme adsorption from aqueous solution was determined as 365.16mg/g at pH 10.0 and 1.25mg/mL initial concentration of lysozyme, while the non-specific adsorption of lysozyme was very low (4.21mgg−1 sorbent). We concluded that Bent-Trp microcomposite affinity sorbents could be repeatedly applied for lysozyme adsorption without significant losses in the adsorption capacity.
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