Adrenergic cell group in rostral ventrolateral medulla of cat: its correlation with central chemoreceptors

Abstract

We have proposed a hypothesis that secondary neurons mediating central respiratory chemoreception are not restricted to the medullary superficial layer. This idea was further examined in the present physiological and morphological studies. We identified the ‘S’ area, i.e. the medullary surface area where cold blockade produced apnea, in anesthetized, spontaneously breathing cat. We then evaluated how the apnea was modified by injection of CO 2-saturated saline into the vertebral-basilar artery. The CO 2 injection caused immediate reappearance of respiratory rhythm, although intensity of inspiratory activity was smaller than in the control. This incomplete recovery suggests that the responsive structure extends deeply below the surface. The extent of the cell group underlying the ‘S’ area was evaluated by morphological study. Medullary transverse sections including the ‘S’ area were treated with three distinct antisera against phenylethanolamine N-methyltransferase (PNMT), dopamine-β-hydroxylase (DBH), and 5-hydroxytryptamine (5-HT). The PNMT-labelled cell group was found to exactly underlie the ‘S’ area, although the DBH- and the 5-HT-labelled cell groups did not show close topographic correlation with the ‘S’ area. PNMT cells were located in the region ventral to retrofacial nucleus within 0.5–1.5 mm depth beneath the surface. These results suggest that adrenergic cells in the rostral ventrolateral medulla are important candidates for secondary neurons mediating central respiratory chemoreception.