ADMINISTRATION OF PAPAYA LEAF (Carica papaya L.) ETHANOL EXTRACT TO YOUNG ADULT MICE (Mus musculus) LEADS TO LOWER SPERMATOZOA QUALITY THAN CONTROLS

Abstract

Increasing population is one of the problems faced in Indonesia. Men's participation and knowledge of contraception is still lacking. Papaya leaves are thought to have an antifertility effect due to the content of secondary metabolites, namely flavonoids, alkaloids, saponins, tannins and papain enzymes that have estrogenic effects that can interfere with hormone regulation in the hypothalamic-pituitary-gonadal axis. This study used an experimental study with a post-test only control group design. The samples used were Swiss Webster strain male mice (Mus musculus) aged 2-3 months with a body weight of 20-30 grams as many as 36 mice divided into 2 groups. The control group (P0) was given 0.5 ml of 3% Tween and the treatment group (P1) was given 0.21 mg/gram BW ethanol extract of papaya leaves (Carica papaya L.) for 36 days orally. The data obtained were analyzed using the Independent Sample T-test. This study showed the results: (1) the average concentration of spermatozoa in the control group (107.6 million/ml) was higher than the treatment group (83.0 million/ml) with p=0.000; (2) the mean of rapidly progressive spermatozoa motility in the control group (41.0 µm/sec) was higher than the treatment group (19.0 µm/sec) with p= 0.000; (3) the average viability of spermatozoa in the control group (87.7%) was higher than the treatment group (71.9%) with p=0.000; (4) the normal morphological mean of the spermatozoa treatment group (81.6%) was higher than the normal morphological mean of the treatment group (38.1%) with p=0.000. Keywords: Male Contraception; Papaya Leaf Extract; Spermatozoa Quality.