Abstract
BackgroundThe purpose of this study is to investigate the clinical effects of platelet-rich plasma (PRP) and adipose-derived mesenchymal stem cell (MSC) as the fundamental treatment of osteoarthritis (OA).MethodsTwenty four Beagle dogs were used as cranial cruciate ligament transection models. The dogs were divided into four groups (n = 6) according to the intra-articular injection materials: the control group with phosphate-buffered saline (PBS), the PRP group with PRP, the MSC group with MSCs emerged in PBS, and the MSC and PRP co-treatment (MP) group with MSCs and PRP.ResultsLameness score, focal compression strength, articular extracellular matrix (ECM) compositions, histopathology, and real-time PCR were used to evaluate the effects of PRP and MSCs on canine OA. In the order of MP, PRP, and MSC group, these all showed positive effects on the evaluated categories. The lameness scores were lower, and the focal compression strengths of the affected femoral articular surface cartilages were higher than those in the OA control group. Also, the inflammatory changes, when evaluated with Mankin scoring and histomorphologic examination, were significantly ameliorated with the treatment of PRP and/or MSCs. The glycosaminoglycan and collagen composition of extracellular matrix was more favorable in the test groups. The ECM-related genes significantly increased through the up-regulation, while the protein expressions of inflammatory cytokines were decreased through the inhibitory effects of PRP and MSCs on chondrocyte apoptosis and inflammatory cytokines.ConclusionsTaken together, this study suggests that PRP and MSCs treatments have a beneficial effect on OA via the stimulation of ECM synthesis and chondrocyte proliferation and via the inhibition of inflammatory reaction.Electronic supplementary materialThe online version of this article (doi:10.1186/s13018-016-0342-9) contains supplementary material, which is available to authorized users.
Highlights
The purpose of this study is to investigate the clinical effects of platelet-rich plasma (PRP) and adipose-derived mesenchymal stem cell (MSC) as the fundamental treatment of osteoarthritis (OA)
The lameness score was significantly decreased at 2 months and at 3 months after treatment in the PRP group and MSC and PRP co-treatment (MP) group when compared with the previous treatment, respectively (Fig. 1)
When compared with control group, it was found that the Mankin score significantly reduced with the treatment of all three test materials on the femoral and tibial articular cartilages
Summary
The purpose of this study is to investigate the clinical effects of platelet-rich plasma (PRP) and adipose-derived mesenchymal stem cell (MSC) as the fundamental treatment of osteoarthritis (OA). Osteoarthritis (OA) is the most common clinical syndrome of joint pain and dysfunction, accompanied by varying degrees of functional limitation and the reduced quality of life. Because it is mostly irreversible and progressive, OA joint loses their cartilage layer [1]. Therapies for the OA commonly focused on the palliation of pain and discomfort, improvement on functional movement, and prevention of further degeneration.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
