Abstract
ADMSCs were isolated from subcutaneous adipose tissue, characterized and cultured in vitro. GFP-labeled ADMSCs can grow and proliferate well on the Atelocollagen scaffolds, and induced by 5-aza the cells can differentiate into cardio-like cells. 3D cultured ADMSCs on Atelocollagen scaffolds were transplanted into mice ischemia myocardium, and have good biocompatibility with host cardio tissue.
Highlights
Over the past decades, a variety of stem cells have been used in clinical trials
adipose tissue-derived mesenchymal stem cells (ADMSCs) and Atelocollagen scaffolds complex were fixed with 4% paraformaldehyde for 30 min, treated with 0.3% Triton for 10 min and blocked with goat serum for 30 min
We demonstrated for the first time that ADMSCs can attach, proliferate and differentiate on Atelocollagen scaffolds, suggesting that this biomaterial may be used in 3D printing to print out suitable scaffolds for cardio repair according to infarct size, shape, etc
Summary
A variety of stem cells have been used in clinical trials. Stem cell transplantation, has been a new method for the treatment of myocardial infarction (MI), a severe clinical condition. Three types of biological materials have mainly been used for myocardial tissue engineering research: natural materials, synthetic materials, and composite materials. Natural materials mainly include collagen, coral, amino dextran, chondroitin sulfate, gelatin, sodium alginate, fibrin gel, etc. Synthetic materials refer to polyurethane, polyglycolic acid (PGA), polylactic acid (PLA),
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