Adhesion, proliferation and ALP activity of osteoblasts on porous Ti processed by laser solid forming

Abstract

Laser Solid Forming (LSF) is an advanced fabrication technology, and has rapid, accurate, and economical advantages compared with traditional processing methods. Fabrication of biological materials using LSF can personalizes design and production for the individual patient to meet the individual needs of different patients. Therefore, fabrication of porous metallic biomaterials using LSF has great application prospects. In this paper, our objective is to study the biocompatibility of osteoblasts on porous Ti processed by laser solid forming. First the porous Ti materials was built by Laser Solid Forming, and machined to testing samples, SD rat osteoblasts were seeded on porous Ti surface. Adhesion, proliferation and ALP activity of osteoblasts were measured at different time, and all samples were observed under SEM for cell morphologies. Dense sand-blasted Ti sheets were used as the controls. All data were statistically analyzed. The results show that the cell numbers on porous Ti were higher than those on nonporous Ti sheets after 2 and 3 hours of culture(P<0.05). At 4 and 7 days, cell proliferation and ALP activity were significantly higher on porous Ti than those on the controls (P<0.05). All results indicated that the porous Ti processed by LSF possesses good biocompatibility to osteoblasts and may stimulate original cell adhesion, proliferation and ALP activity.Laser Solid Forming (LSF) is an advanced fabrication technology, and has rapid, accurate, and economical advantages compared with traditional processing methods. Fabrication of biological materials using LSF can personalizes design and production for the individual patient to meet the individual needs of different patients. Therefore, fabrication of porous metallic biomaterials using LSF has great application prospects. In this paper, our objective is to study the biocompatibility of osteoblasts on porous Ti processed by laser solid forming. First the porous Ti materials was built by Laser Solid Forming, and machined to testing samples, SD rat osteoblasts were seeded on porous Ti surface. Adhesion, proliferation and ALP activity of osteoblasts were measured at different time, and all samples were observed under SEM for cell morphologies. Dense sand-blasted Ti sheets were used as the controls. All data were statistically analyzed. The results show that the cell numbers on porous Ti were higher than those o...