Abstract
The purpose of this study was to measure the amount of adsorption of various salivary proteins to a resin composite having various amounts of surface pre-reacted glass-ionomer (S-PRG) fillers, and to make a comparative study of the adherence of S. mutans to the resin composite covered by various salivary proteins. We experimentally produced resin composites (S-PRG resin) having the basic composition of Bis-GMA/TEGDMA and various amount of the S-PRG fillers ranging between 0 - 60 wt%. Each S-PRG resin block was soaked in 5 kinds of components found in salivary fluid (Mucin 1, Lactoferrin, IgA, Cystatin C, and Lysozyme), and the amount of adsorption was measured by use of a spectrophotometer. The amount of the adsorption of salivary Mucin 1 was higher than that of any other salivary component tested regardless of the percentage of the S-PRG filler. In the case of salivary Lysoxyme used for coating, the amount of its adsorption increased with an increase in the percentage of the S-PRG filler. In addition, resin blocks coated with various salivary proteins were incubated at 37℃ for 2 hours with radio-labeled S. mutans for a quantitative adherence test. Labeled bacteria that adhered to the resin blocks were collected by using an automatic sample combustion system and a liquid scintillation counter. The absorbed salivary components, especially Mucin 1 and Lysozyme, inhibited the adhesion of S. mutans to the S-PRG resin; however, these changes were generally directional rather than statistically significant.
Highlights
The resin composite restoration is widely used due to the spread of the concept of Minimal Intervention (MI) advocated by the Federation Dentaire Internationale (FDI) [1] [2]
The purpose of this study was to measure the amount of adsorption of various salivary proteins to a resin composite having various amounts of surface pre-reacted glass-ionomer (S-PRG) fillers, and to make a comparative study of the adherence of S. mutans to the resin composite covered by various salivary proteins
The S-PRG filler was found to release inorganic elements such as Al3+, Sr2+, Na+, SiO23−, and BO33− [11]. In this present study we examined the adsorption of various salivary components to S-PRG resins and determined how each component affected the adhesion of S. mutans to the S-PRG resin surface
Summary
The resin composite restoration is widely used due to the spread of the concept of Minimal Intervention (MI) advocated by the Federation Dentaire Internationale (FDI) [1] [2]. It is necessary to think about the risk of secondary caries after restoration because dental plaque is formed on the surface of a resin composite [3]. The surface components of a resin composite may play an important role in the adherence of bacteria to the composite. Plaque does not form directly on the surfaces of a tooth or resin composite but requires first the adsorption of proteins to such a surface, resulting in a layer known as the acquired pellicle. The composition of dental pellicles is mainly based on the selective adsorption of salivary proteins [5] [6]
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