Abstract
A sulfuric acid–EDTA method for extracting ATP from soil and marine sediments was devised. The efficacy of this method is compared to the boiling Tris extraction procedure. Efficiency of ATP recovery from solid substrates typically ranged between 81–94% for the sulfuric acid–EDTA procedure in comparison to a recovery of 3–6% using boiling Tris buffer. The presence of various ions in the acid extract, however, inhibited light emission in the luciferin–luciferase measurement of ATP. Addition of EDTA to the extract reduced but did not eliminate the ionic interference. An internal ATP standard was thus used to determine the magnitude of the residual interference.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
