Abstract
The present study examined the effects that blocking local adenosine and nitric oxide production has on skeletal muscle interstitial pH and H+ during hypoxia. Eight anesthetized rats had two microdialysis fibers inserted into the gastrocnemius muscle of each leg and perfused with Ringers (control) or 500 μM AOPCP (5′ectonucleotidase inhibitor) plus 10 mM L‐NAME (NOS inhibitor). Following baseline collections (20 min with saline, Baseline 1), all the probes were perfused with the both blockers for (20 min, Baseline 2) followed by 20 min of hypoxia (inhalation of 10.5% O2) and recovery. The perfusion of the blockers resulted in a decrease (P<0.05) in skeletal muscle interstitial pH and a reciprocal increase (P<0.05) in interstitial H+during normoxia (Baseline 2) and these changes were further exaggerated during hypoxia as well as recovery (Figures)1. These data clearly demonstrate that blocking local vasodilators most likely resulted in a decrease in local tissue blood flow and an accumulation of H+, which is further elevated under anaerobic conditions such as hypoxia.
Published Version
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