Addressing global food insecurity: Soil-applied zinc oxide nanoparticles promote yield attributes and seed nutrient quality in Glycine max L.

Abstract

Consumed globally, oilseeds serve as a major source of proteins and oils in human and animal nutrition, supporting global food security. Zinc (Zn) is an essential micronutrient critical for oil and protein synthesis in plants. In this study, we synthesized three distinct sized zinc oxide nanoparticles (nZnO: 38 nm = S [small], 59 nm = M [medium], and > 500 nm = L [large], and assessed the potential effects of varied particle sizes and concentrations (0, 50, 100, 200, and 500 mg/kg-soil) on seed yield attributes, nutrient quality and oil and protein yield in soybean (Glycine max L.) grown for a full lifecycle of 120 days, and compared with soluble Zn2+ ions (ZnCl2) and water-only controls. We observed particle size- and concentration-dependent influence of nZnO on photosynthetic pigments, pod formation, potassium and phosphorus accumulation in seed, and protein and oil yields. Overall, soybean showed significant stimulatory responses to nZnO-S for most of the parameters tested compared to nZnO-M, nZnO-L, and Zn2+ ions treatments up to 200 mg/kg, suggesting the potential for small size nZnO to improve seed quality and production in soybean. At 500 mg/kg, however, for all endpoints (except for carotenoids and seed formation) toxicity was observed with all Zn compounds. Further, TEM analysis of seed ultrastructure indicated potential alterations in seed oil bodies and protein storage vacuoles at a toxic concentration (500 mg/kg) of nZnO-S compared to control. These findings suggest 200 mg/kg as an optimal dose for the smallest size nZnO-S (38 nm) to significantly improve seed yield, nutrient quality, and oil and protein yield, paving a path for addressing global food insecurity using small sized nZnO as a novel nano-fertilizer to promote crop yield and nutrient quality, in soil-grown soybean.