Abstract

Figure S2. Fluorescence and immunofluorescence micrographs of transverse resin sections of resin canals in OW labelled with LM5. Fluorescence micrographs of a normal resin canal (a) and a pair of traumatic resin canals (c) and immunofluorescence micrographs the same normal resin canal (b) and pair of traumatic resin canals (d). The fluorescence micrographs shows the distribution of lignin and other autofluorescent materials. The epithelial and parenchyma walls of both normal and traumatic resin canals and the tracheid walls show autofluorescence. The thin-walled epithelial cells (E) in both normal and traumatic resin canals are sparsely labelled with LM5. The walls of the parenchyma cells (P) in both types of canals are brightly labelled. The ray (R) cell walls are also brightly labelled. Sections were from Tree 1 and the micrographs obtained using a Zeiss confocal microscope. Scale bar: 20 μm. (PDF 312 kb)

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