Abstract
This paper develops a practical and useful computer control scheme to control the specific growth rate as accurately as possible by measuring the released protons in anaerobic alcohol fermentation. In the case of current study, most of the released protons are due to the uptake of cationic ammonium ion via the conversion: NH4+ → NH3(cell) + H+. Correlating the Proton Production (PP) and the Proton Production Rate (PPR) with specific growth rate (μ) proved PP as a better measured variable. Using a simple adaptive control algorithm, μ was successfully controlled in a Zymomonas mobilis fed-batch culture.
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