ADAPTATION TO MULTISTRESS IN VITRO OF MYCOBACTERIUM TUBERCULOSIS STRAINS OF EAST-ASIAN AND EURO-AMERICAN LINEAGES, DIFFERING IN DRUG RESISTANCE

Abstract

In order to study the in vitro adaptation of M.tuberculosis strains of various phylogenetic lineages and drug resistance under multistress conditions created by a lack of nutrients, reactive forms of nitrogen and oxygen, 17 strains of M.tuberculosis were studied (10 strains of the East Asian lineage of the Beijing sublineage and 7 strains Euro-American lineage). We studied the culture yield, the state of M.tuberculosis cells in culture and the secretome when cultivating strains under conditions of multistress, nitrosative stress and in optimal conditions. It has been established that M. tuberculosis strains of frequently SITs with a sensitive genotype or with widespread mutations, in contrast to strains with rare mutations and with an expanded spectrum of phenotypic drug resistance, adapt well to stress conditions in vitro. M.tuberculosis strains of the Beijing sublineage adapt to stress at an earlier time than M.tuberculosis of the Euro-American lineage; this was especially evident in the multistress model.