Abstract
A horizontal DS chamber for TLC has been adapted for planar electrochromatography in closed system. A test dye mixture was separated on pre-wetted RP-8 and RP-18 chromatographic plates with acetonitrile-buffer as mobile phase. A potential of 2 kV was applied to a 10-cm plate to create the electric field. Two modes of electrochromatogram development were applied — in open and closed (pressurized) systems. The disadvantages of the open system — evaporation of the mobile phase from the plate and excessive flow of mobile phase to the surface of the adsorbent layer during development — were eliminated by use of the closed system. Highly reproducible retention was achieved during planar electrochromatography in the closed system when the adsorbent layer of the plate was pre-wetted and equilibrated with a solution of the mobile phase. Examples are presented of electrochromatograms obtained from the test dye mixture under different operating conditions. Separation efficiency in planar electrochromatography was hi...
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