Adaptation mechanisms of leaf vein traits to drought in grassland plants

Abstract

Leaf veins play an important role in water transport, and are closely associated with photosynthesis and transpiration. Resource heterogeneity in the environment, particularly in water resources, causes changes in leaf vein structure and function, thereby affecting plant growth and community assemblages. Therefore, it is necessary to explore the spatial variation and evolutionary mechanisms of leaf veins in natural communities. Natural communities are composed of dominant and non-dominant species. However, few studies to date have explored the trait variation of dominant and non-dominant species on a large scale. In this study, we set up 10 sampling sites along the water gradient (from east to west) in the Loess Plateau of China, and measured and calculated the vein density (vein length per unit area, VLA), vein diameter (VD), and vein volume ratio (VVR) of 173 species, including dominant and non-dominant species. The mean values of VLA, VD, and VVR were 10.95 mm mm−2, 22.24 μm, and 3%, respectively. VD and VVR of the dominant species were significantly higher than those of the non-dominant species. Unexpectedly, there was no significant change in the VLA with the water gradient, although the VD increased with drought. Leaf vein traits did not change significantly with evolution. There was a significant trade-off between VLA and VD. Our findings demonstrate that the response of veins to environmental changes is dependent on the degree of drought and provide new insights for further large-scale studies.