Abstract
BackgroundNitrogen-based nutrients are the main factors affecting rice growth and development. Root systems play an important role in helping plants to obtain nutrients from the soil. Root morphology and physiology are often closely related to above-ground plant organs performance. Therefore, it is important to understand the regulatory effects of nitrogen (N) on rice root growth to improve nitrogen use efficiency.ResultsIn this study, changes in the rice root traits under low N (13.33 ppm), normal N (40 ppm) and high N (120 ppm) conditions were performed through root morphology analysis. These results show that, compared with normal N conditions, root growth is promoted under low N conditions, and inhibited under high N conditions. To understand the molecular mechanism underlying the rice root response to low and high N conditions, comparative proteomics analysis was performed using a tandem mass tag (TMT)-based approach, and differentially abundant proteins (DAPs) were further characterized. Compared with normal N conditions, a total of 291 and 211 DAPs were identified under low and high N conditions, respectively. The abundance of proteins involved in cell differentiation, cell wall modification, phenylpropanoid biosynthesis, and protein synthesis was differentially altered, which was an important reason for changes in root morphology. Furthermore, although both low and high N can cause nitrogen stress, rice roots revealed obvious differences in adaptation to low and high N.ConclusionsThese results provide insights into global changes in the response of rice roots to nitrogen availability and may facilitate the development of rice cultivars with high nitrogen use efficiency through root-based genetic improvements.
Highlights
Nitrogen-based nutrients are the main factors affecting rice growth and development
The morphology and physiology of rice roots are closely related to nitrogen uptake and utilization (Zhang et al 2009; Lynch 2013)
The molecular regulatory mechanisms underlying the morphological and physiological acclimation to different nitrogen levels remain largely unknown. Understanding these mechanisms is of great importance for the scientific community involved in research on rice biology and practical rice breeders, as knowledge of rice root responses to nitrogen availability enables the breeding of rice with high nitrogen use efficiency
Summary
Nitrogen-based nutrients are the main factors affecting rice growth and development. It is important to understand the regulatory effects of nitrogen (N) on rice root growth to improve nitrogen use efficiency. Changes in nitrogen availability affect root morphology and physiology (Xu et al 2012). The molecular regulatory mechanisms underlying the morphological and physiological acclimation to different nitrogen levels remain largely unknown. Understanding these mechanisms is of great importance for the scientific community involved in research on rice biology and practical rice breeders, as knowledge of rice root responses to nitrogen availability enables the breeding of rice with high nitrogen use efficiency
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
