Abstract
The objective of this study was to use the same concentrations of aluminum (Al) and manganese (Mn) detected previously in groundwater above those permitted by Brazilian law and assess their cytotoxic and genotoxic effects in hamster ovary cell lines and their mutagenic effects through the Salmonella microsome assay. Chinese hamster ovary (CHO) and CHO-XRS5 cells were treated with different concentrations of Al and Mn (0.2 to 2.0 mg/L and 0.1 to 3.0 mg/L, respectively). The Ames test was used to analyze the concentrations of Al and Mn ranging from 0.025 to 1.0 mg/L and 0.0125 to 1.5 mg/L, respectively. Both metals showed cytotoxic effects on both cell lines and two bacterial strains (TA98 and TA100). The genotoxic effects of the highest concentrations of Al and Mn in cell lines showed nuclear buds, micronuclei, and DNA damage; however, none of the concentrations showed a positive mutagenic response in the Ames test. This is one of the few studies to demonstrate the cytotoxic effects of Al and Mn through the Ames test. In addition, the metals caused genomic instability in cell lines. Therefore, this study may help hasten the review of established regulatory standards for human consumption of groundwater.
Highlights
IntroductionExposure to metals in the environment at concentrations above of the values considered safe by legislation can adversely affect organisms [1,2,3]
To identify the potential effects caused by Al and Mn in the populations of these cities, the objective of this study was to use the same concentrations of Al and Mn detected previously and assess their cytotoxic and genotoxic effects in hamster ovary cell lines and their mutagenic effects through the Salmonella microsome assay
The viability of Chinese hamster ovary (CHO) and CHO-XRS5 cells was reduced upon exposure to Al and Mn of CHO and CHO-XRS5 cells was reduced upon exposure to Al and Mn for 24 andThe
Summary
Exposure to metals in the environment at concentrations above of the values considered safe by legislation can adversely affect organisms [1,2,3]. Metal contamination in the environment occurs from both natural sources, such as rocks and volcanoes, and anthropogenic sources, such as through industrial processes, mining, pesticides, medication, and water treatment [4,5]. Contaminated food and drinking water are the most common sources of human exposure to metals [6,7]. Food contamination is mainly caused by the use of pesticides containing metals in agriculture, whereas contamination of water is mainly caused by inadequate disposal of these metals in the soil due to agricultural, industrial, and landfill activities, which can contaminate surface and groundwater through percolation and leaching processes [8]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
