Abstract
In the culture of human keratinocytes (neonatal foreskin cells), the acute responses to calcium stimulus were examined by observing the fluctuation in the projected areas of individual cells. The cells showing the suppressed fluctuation (denoted as calcium-responding cells) appeared when Ca 2+ was injected to medium at 0.6 or 1.2 mol/m 3 (normally 0.15 mol/m 3) in the first passage during a series of the successive cultures. Moreover, it was found that the ratio of calcium-responding cells decreased in a linear manner with an increase in the cumulative number of population doublings, suggesting that the calcium responsiveness of the keratinocytes can be correlated with the extent of cellular differentiation.
Published Version
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